| Lane | Athlete (Yr.) Team |
Yr. | Seed | Note |
|---|---|---|---|---|
| 2 | HOLIWAY, Eleanor (SO) Smith-Cotton High School |
SO | 1:04.04 | Verified |
| 3 | HOLLOWAY, Rebecca (FR) Jefferson City High School |
FR | 58.22 | Verified |
| 4 | DELK, Lily (SR) Helias Catholic High School |
SR | 53.30 | Verified |
| 5 | HOFEDITZ, Riley (FR) Hickman High School |
FR | 57.42 | Verified |
| 6 | MILLER, Elizabeth (FR) Jefferson City High School |
FR | 1:00.62 | Verified |