| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | SPELLMEYER, Jaden | SO | Smith-Cotton High School | 1:09.93 | Verified |
| 3 | MORRISEY, Iris | SO | Helias Catholic High School | 1:06.86 | Verified |
| 4 | JOHNSON, Maria | JR | Battle High School | 1:06.00 | Unverified: |
| 5 | PISTEL, Kambry | JR | Capital City High School | 1:06.78 | Unverified: |
| 6 | JOHNSTON, Izabell | SR | Jefferson City High School | 1:08.33 | Verified |
| 7 | KENSINGER, Emmaline | JR | Smith-Cotton High School | 1:11.12 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | FEDENIA, Aida | FR | Rock Bridge High School | 1:04.89 | Unverified: |
| 2 | LEIGH, Sophia | JR | Hickman High School | 1:02.12 | Verified |
| 3 | GAINES, Journey | FR | Battle High School | 1:01.05 | Verified |
| 4 | EDWARDS, Mikah | SR | Helias Catholic High School | 56.63 | Verified |
| 5 | MORRIS, Seleena | SR | Jefferson City High School | 59.99 | Verified |
| 6 | STEMMONS, Sy'Rae | SO | Hickman High School | 1:01.81 | Verified |
| 7 | MILLER, Lauren | SO | Rock Bridge High School | 1:02.24 | Verified |
| 8 | HESS, Layla | SO | Capital City High School | 1:05.09 | Verified |