| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | WHITEHEAD, Mikiaha | FR | Jefferson City High School | 1:19.14 | Verified |
| 4 | PARKER, Emily | JR | Hickman High School | 1:15.00 | Unverified: |
| 5 | SALL, Elizabeth | FR | Hickman High School | 1:16.08 | Verified |
| 6 | POPOVA, Alina | FR | Smith-Cotton High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | NDEGWA, Elizabeth | JR | Capital City High School | 1:13.50 | Verified |
| 2 | BROKER, Erin | FR | Helias Catholic High School | 1:09.23 | Verified |
| 3 | LUTZ-JENKINS, Kennedie | FR | Capital City High School | 1:08.20 | Verified |
| 4 | SCHULTZ, Brianna | SR | Battle High School | 1:02.80 | Verified |
| 5 | EVANS, Mikhaya | JR | Battle High School | 1:04.00 | Unverified: |
| 6 | IRELAND, Isabelle | FR | Rock Bridge High School | 1:08.90 | Verified |
| 7 | GOETZ, Emma | SO | Helias Catholic High School | 1:10.12 | Unverified: |
| 8 | KFURI, Julia | FR | Rock Bridge High School | 1:13.60 | Verified |