| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Helias Catholic High School | A | Lanigan | Hoelscher | Neuenswander | Marberry | 45.24 | Verified |
| 2 | Smith-Cotton High School | A | Brewer | Ladd | Lorenzo | Washington | 44.48 | Verified |
| 3 | Hickman High School | A | Milo | Wright | Zink | Thomas | 44.25 | Verified |
| 4 | Rock Bridge High School | A | Amoah-Owusu | TRUITT | Taylor | Ennin | 42.63 | Verified |
| 5 | Jefferson City High School | A | Wilson | Wilson | Wright | Wilson | 42.97 | Verified |
| 6 | Battle High School | A | Holt | Ojo | Horn | Donnelly | 44.29 | Verified |
| 7 | Capital City High School | A | Harrison | Franklin | Mornan | Borde-Koufie | 44.63 | Verified |