| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Capital City High School | A | Bonchonsky | Austin | Robinson | McRoberts | 47.63 | Unverified: |
| 2 | Rock Bridge High School | A | Hood | Grotewiel | Young | Atkinson | NT | Unverified: |
| 3 | Jefferson City High School | A | Barnes | Lowery | Cody | LaViolette Stewart | 44.65 | Unverified: |
| 4 | Battle High School | A | Gordon | Christopher | Rice | Harris | 44.29 | Verified |
| 5 | Smith-Cotton High School | A | Blas | Bunker | Gray | Neal | 44.48 | Verified |
| 6 | Helias Catholic High School | A | Lanigan | Crisostomo | Swan | Jerozal | 45.24 | Verified |
| 7 | Hickman High School | A | Fasken | Frederick | Gould | Hester | 46.25 | Unverified: |