| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Rock Bridge High School | A | Lawal | Szydlowski | Fisher | Dawson | 54.15 | Unverified: |
| 3 | Battle High School | A | Carter | Green | Schultz | Belcher-Avant | 51.00 | Unverified: |
| 4 | Helias Catholic High School | A | Delk | French | Mason | Lock | 49.51 | Verified |
| 5 | Capital City High School | A | Mays | Warren | Kuebler | Bibbs | 50.17 | Verified |
| 6 | Hickman High School | A | Cole | Hofeditz | Redmond-Lilly | Wray | 53.82 | Unverified: |
| 7 | Jefferson City High School | A | O'Donnell | Ingram | Luebbering | Harden | 55.19 | Unverified: |