| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HAUSER, Andrew | JR | Rock Bridge High School | 4:11.72 | Verified |
| 2 | SUMMERALL, Hudson | SR | Rock Bridge High School | 4:31.62 | Verified |
| 3 | PILLIARD, Clay | SR | Smith-Cotton High School | 4:37.82 | Verified |
| 4 | BENNE, Ian | SO | Helias Catholic High School | 4:38.05 | Verified |
| 5 | SCHOFIELD, Brock | SR | Capital City High School | 4:38.54 | Verified |
| 6 | DOOLITTLE, Noah | FR | Helias Catholic High School | 4:39.49 | Verified |
| 7 | STUMBAUGH, Seth | JR | Jefferson City High School | 4:39.76 | Verified |
| 8 | MUHLENBRUCK, Elliot | SR | Hickman High School | 4:54.99 | Verified |
| 9 | BRINKMAN, Joshua | SO | Hickman High School | 5:07.22 | Verified |
| 10 | GERBES, Jameson | FR | Jefferson City High School | 5:21.79 | Verified |
| 11 | TUYISENGE, Eric | SR | Battle High School | 5:22.84 | Verified |
| 12 | SILL, Noah | SO | Battle High School | 5:50.44 | Verified |
| 13 | ZALPA, Julio | JR | Smith-Cotton High School | 5:52.52 | Verified |
| 14 | NOBLE, Parker | SR | Capital City High School | NT | Unverified: |