| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Rock Bridge High School | A | Richardson | Sexten | Walker | Ford | 9:51.17 | Verified |
| 2 | Helias Catholic High School | A | Verslues | Mathews | Morrisey | Dollar | 10:28.57 | Verified |
| 3 | Jefferson City High School | A | Smith | Schnipper | Cordes | Kinsey | 10:38.22 | Verified |
| 4 | Capital City High School | A | Kemna | Kirby | Robben | Schulte | 10:53.86 | Verified |
| 5 | Smith-Cotton High School | A | Carrico | Kuehner | Gonzalez | Newkirk | 11:11.00 | Verified |