| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 15 | KIM, Matthew | FR | Rock Bridge High School | 4:34.27 | Verified |
| 16 | HUDSON, Cook | FR | Rock Bridge High School | 4:35.47 | Verified |
| 17 | HARLAN, Thomas | JR | Capital City High School | 4:54.36 | Verified |
| 18 | VOEGELI, Mason | JR | Helias Catholic High School | 5:08.05 | Verified |
| 19 | BUXTON, Coen | SO | Battle High School | 5:12.49 | Verified |
| 20 | HALL, Mikel | FR | Battle High School | 5:13.43 | Verified |
| 21 | WIEBERG, Jonathan | SR | Helias Catholic High School | 5:14.81 | Verified |
| 22 | SHARP, Joshua | SR | Capital City High School | 5:20.84 | Verified |
| 23 | YOON, Yule | SO | Hickman High School | 5:56.19 | Verified |
| 24 | WOODRUFF, Noah | FR | Jefferson City High School | 5:58.72 | Verified |
| 25 | ELLISON, Indiana | SO | Smith-Cotton High School | 6:05.59 | Verified |
| 26 | RIBIAT, Phoenix | SO | Hickman High School | 6:22.46 | Verified |
| 27 | CONTRERAS, Jorge | JR | Smith-Cotton High School | 6:37.00 | Verified |