| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | FORD, Carolyn | SR | Rock Bridge High School | 5:01.10 | Verified |
| 2 | WALKER, Mae | FR | Rock Bridge High School | 5:16.60 | Verified |
| 3 | EASTMAN, Ellie | SO | Hickman High School | 5:30.59 | Verified |
| 4 | BUKER, Danielle | SR | Helias Catholic High School | 5:38.13 | Verified |
| 5 | CARRICO, Lindsey | FR | Smith-Cotton High School | 5:49.90 | Verified |
| 6 | HARRIS, Allison | SR | Battle High School | 5:50.43 | Verified |
| 7 | CORDES, Makayla | JR | Jefferson City High School | 6:07.10 | Verified |
| 8 | JONES, Adelaide | JR | Jefferson City High School | 6:24.50 | Verified |