| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 9 | CRANE, Amelie | SO | Rock Bridge High School | 5:35.01 | Verified |
| 10 | GILLIS, Peyton | JR | Rock Bridge High School | 5:46.43 | Verified |
| 11 | KEENOY, Natalie | FR | Hickman High School | 6:02.17 | Verified |
| 12 | HUCKSTEP, Savanna | SR | Helias Catholic High School | 6:22.53 | Verified |
| 13 | ROLING, Allison | FR | Helias Catholic High School | 6:35.78 | Verified |
| 14 | SAN JUAN RODRIGUEZ, Evelyn | JR | Battle High School | 6:40.53 | Verified |
| 15 | GARDNER, Makayla | FR | Jefferson City High School | 6:46.83 | Verified |
| 16 | BUKOSKI, Emma | FR | Hickman High School | 6:47.00 | Verified |
| 17 | MCDONALD, Arabelle | SO | Battle High School | 7:00.79 | Verified |
| 18 | JOHNSON, Payton | FR | Smith-Cotton High School | 7:12.35 | Verified |
| 19 | BERNSKOETTER, Addison | FR | Capital City High School | 7:14.54 | Verified |
| 20 | CHEN, Ria | SR | Capital City High School | 7:15.24 | Verified |