| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Rock Bridge High School | A | Bailey | Holt | Amoah-Owusu | TRUITT | 1:35.00 | Unverified: |
| 2 | Helias Catholic High School | A | Lanigan | Knernschield | Malmstrom | Marberry | 1:33.30 | Verified |
| 3 | Smith-Cotton High School | A | Grubb | Lorenzo | Smith | Washington | 1:32.40 | Verified |
| 4 | Jefferson City High School | A | Wilson | Wilson | Wilson | Wright | 1:31.14 | Verified |
| 5 | Hickman High School | A | Zink | DiBlasi | Milo | Wright | 1:32.39 | Verified |
| 6 | Capital City High School | A | Harrison | Franklin | Mornan | Borde-Koufie | 1:33.02 | Unverified: |
| 7 | Battle High School | A | Owusu | Donnelly | Karara | Lakes | 1:34.14 | Verified |