| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Rock Bridge High School | A | Hood | Grotewiel | Scott | Atkinson | 1:39.42 | Unverified: |
| 2 | Jefferson City High School | A | Barnes | LaViolette Stewart | Lowery | Holloway | 1:36.00 | Unverified: |
| 3 | Smith-Cotton High School | A | Alicea | Askerov | Clark | Gray | 1:34.85 | Unverified: |
| 4 | Helias Catholic High School | A | Lanigan | Crisostomo | Swan | Jerozal | 1:33.30 | Verified |
| 5 | Hickman High School | A | Brown | Fasken | Frederick | Gould | 1:34.39 | Unverified: |
| 6 | Battle High School | A | Nelson | Harris | Rice | McCray | 1:35.00 | Unverified: Winnetonka Invitational |
| 7 | Capital City High School | A | Bonchonsky | Austin | Robinson | Johnson | 1:37.02 | Verified |