| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Smith-Cotton High School | A | Bell | Taylor | Mackin | Washington | 1:54.50 | Verified |
| 2 | Battle High School | A | Gaines | Brookins | Jones | Anye | 1:47.94 | Verified |
| 3 | Hickman High School | A | Maland | Smith | Stemmons | Leigh | 1:46.90 | Verified |
| 4 | Helias Catholic High School | A | Phillips | Struemph | Harrison | Edwards | 1:44.72 | Verified |
| 5 | Jefferson City High School | A | Pemberton | Graham | Galbreath | Morris | 1:46.09 | Verified |
| 6 | Rock Bridge High School | A | Parsons | Miller | Gerrish | Fedenia | 1:47.33 | Verified |
| 7 | Capital City High School | A | Cris-Kargbo | Whitley | Pistel | Hess | 1:53.72 | Verified |