| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Rock Bridge High School | A | Szydlowski | Fisher | Kfuri | Ireland | 1:54.33 | Unverified: |
| 3 | Capital City High School | A | Warren | Ndegwa | Lutz-Jenkins | Bibbs | 1:53.72 | Verified |
| 4 | Helias Catholic High School | A | Jahnsen | Goetz | Vallon | Marke | 1:44.72 | Verified |
| 5 | Battle High School | A | Glay | May | Carter | Evans | 1:52.00 | Unverified: |
| 6 | Hickman High School | A | Chang | Goodman | Redmond-Lilly | Sall | 1:53.90 | Unverified: |
| 7 | Jefferson City High School | A | O'Donnell | Ingram | Luebbering | Harden | 2:00.00 | Unverified: |