| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Kennett High School | A | Flores | Boatman | Shields | Henson | DNS | Verified |
| 2 | Ste. Genevieve High School | A | Vogt | Amschler | Butcher | Smith | 3:47.03 | Verified |
| 3 | Arcadia Valley High School | A | Held | Erbland | Mueller | McCallister | 3:36.23 | Verified |
| 4 | Dexter High School | A | Kyle | Bell | Farmer | Stone | 3:31.85 | Verified |
| 5 | Potosi High School | A | Sprous | Cross | Kanan | Brabham | 3:33.68 | Verified |
| 6 | Notre Dame (Cape Girardeau) High | A | Pfeifer | Eftink | Le | Dow | DNS | Verified |
| 7 | Kelly High School | A | Hileman | Burleson | Propst | Shoaf | 3:47.85 | Verified |
| 8 | Doniphan High School | A | Emmons | Schroeder | Clubbs | Owen | 3:49.63 | Verified |