| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 4 | Perryville High School | A | Bohnert | Garris | Hoeckele | Brown | 3:54.15 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | DeSoto High School | A | Bourn | Missey | SISSON | Patterson | 3:41.55 | Verified |
| 2 | Farmington High School | A | Randazzo | Woodson | Logan | Fuller | 3:35.86 | Verified |
| 3 | Central (Cape Girardeau) High Sc | A | Edwards | Brooks-Davis | Eanes | Rusten | 3:33.81 | Verified |
| 4 | Hillsboro High School | A | Marchetti | Brown | Voyles | Holland | 3:23.25 | Verified |
| 5 | Festus High School | A | Anderson | Bates | Branch | Reese | 3:27.52 | Verified |
| 6 | Windsor (Imperial) High School | A | Becker | Harman | Johnson | Hurst | 3:34.23 | Verified |
| 7 | Affton High School | A | Lovelace | Morales | Mitchell | Williams | 3:38.47 | Verified |
| 8 | Sikeston High School | A | Applewhite | WILKINS | HOBBS | Sanders | 3:52.79 | Verified |