| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Kelly High School | A | Thurmond | Essner | Figueroa | King | 4:46.57 | Verified |
| 3 | Kennett High School | A | Farmer | Robinson | Gray | Heeb | DNS | Verified |
| 4 | Ste. Genevieve High School | A | Merklin | Zangaro | Huck | Clements | 4:17.48 | Verified |
| 5 | Dexter High School | A | Essner | Kennedy | Krapf | Turnbo | 4:30.68 | Verified |
| 6 | Potosi High School | A | Sansegraw | Gibson | Coleman | Gibson | 4:41.98 | Verified |
| 7 | Doniphan High School | A | Vierck | Bates | Woods | Maness | DQ | Verified |