| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Affton High School | A | Cousins | Smith | Wilson | Daughtry | 4:31.29 | Verified |
| 2 | Farmington High School | A | Wood | Cassimatis | Burgan | Shuburt | 4:22.57 | Verified |
| 3 | Festus High School | A | DeClue | Hawkins | Holmes | Hawkins | 4:20.58 | Verified |
| 4 | Notre Dame (Cape Girardeau) High | A | Tatum | Eftink | Carr | Givens | 4:14.84 | Verified |
| 5 | Central (Cape Girardeau) High Sc | A | Jackson | Caldwell | Steinberg | Mackins | 4:18.00 | Verified |
| 6 | Hillsboro High School | A | Buettner | Wright | Mora | Wiethop | 4:21.87 | Verified |
| 7 | Windsor (Imperial) High School | A | Dixon | Jarnegan | Mancuso | Mancuso | 4:25.02 | Verified |
| 8 | Sikeston High School | A | Torres | Moore | Shabazz | GIVENS | 4:40.07 | Verified |