| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SCHRAM, Ian | SR | Festus High School | 9:20.99 | Verified |
| 2 | ALLISON, Josh | SR | Hillsboro High School | 9:25.68 | Verified |
| 3 | SEABAUGH, Ryan | SR | Central (Cape Girardeau) High Sc | 9:26.45 | Verified |
| 4 | ALLISON, Jonah | SR | Hillsboro High School | 9:27.22 | Verified |
| 5 | DRIEMEIER, Carson | 9 | Festus High School | 9:27.85 | Verified |
| 6 | MANN, Orville | SR | Hillsboro High School | 9:33.33 | Verified |
| 7 | LEEDS, Jackson | SR | North County High School | 9:51.59 | Verified |
| 8 | KOERBER, Carson | 9 | DeSoto High School | 9:57.26 | Verified |
| 9 | KIMBROUGH, Kaden | JR | Farmington High School | 10:27.27 | Verified |
| 11 | HOECKELE, Preston | SO | Perryville High School | 10:49.06 | Verified |
| 12 | BAUWENS, Clayton | 9 | Perryville High School | 10:50.12 | Verified |
| 13 | KIZER, Noah | SO | Windsor (Imperial) High School | 11:15.87 | Verified |
| 14 | BRADY, Brennan | SO | DeSoto High School | 11:19.03 | Verified |
| 15 | CAIN, Nickolai | SO | Farmington High School | 11:27.83 | Verified |
| 16 | BURTON, Nahshon | SO | Central (Cape Girardeau) High Sc | 11:37.33 | Verified |
| 17 | EARLL, Mason | SO | Affton High School | 11:48.65 | Verified |
| 18 | MOONEY, Tyler | SO | Affton High School | 12:07.71 | Verified |
| 19 | COUCH, Jackson | 8 | Sikeston High School | 13:39.25 | Verified |