| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Dexter High School | A | Kyle | Bell | Farmer | Stone | 8:16.75 | Verified |
| 2 | Potosi High School | A | Gibson | Gibson | Smith | Kanan | 8:29.88 | Verified |
| 3 | Notre Dame (Cape Girardeau) High | A | Dittmer | Givens | Pfeifer | Morris | 8:54.99 | Verified |
| 4 | Central (Park Hills) High School | A | Crocker | Roesch | Weber | Willis | 9:39.90 | Verified |
| 5 | East Prairie High School | A | Rendon | McLemore | Shoemaker | Kenedy | 9:43.21 | Verified |