| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | TRUMAN, Caleb | JR | Farmington High School | 25.93 | Verified |
| 2 | WILKINS, QUENTIN | SO | Sikeston High School | 25.05 | Verified |
| 3 | ANDERSON, Omauri | 9 | Festus High School | 24.11 | Verified |
| 4 | WILLIAMS, Jeremiah | SR | Affton High School | 23.80 | Verified |
| 5 | LITTERST, Caden | SR | Perryville High School | 24.06 | Verified |
| 6 | LEASK, Isaac | SO | Farmington High School | 24.99 | Verified |
| 7 | HOLMAN, Kevin | SO | Windsor (Imperial) High School | 25.84 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MURRAY, Torrance | 9 | Central (Cape Girardeau) High Sc | 23.48 | Verified |
| 2 | HARMAN, Noah | JR | Windsor (Imperial) High School | 22.65 | Verified |
| 3 | JOHNSON, Reece | SR | Festus High School | 22.53 | Verified |
| 4 | HOLLAND, Noah | SR | Hillsboro High School | 21.59 | Verified |
| 5 | VOYLES, Harrison | SR | Hillsboro High School | 22.53 | Verified |
| 6 | THOMAS, Zai'aire | SO | Central (Cape Girardeau) High Sc | 22.65 | Verified |
| 7 | RHYNE, Tyler | SR | Perryville High School | 23.44 | Verified |
| 8 | KELLUM, MALACHI | SR | Sikeston High School | 23.65 | Verified |