| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 4 | FARROW, Katie | JR | Perryville High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | DAVIS, Angelia | SR | Farmington High School | 30.07 | Verified |
| 2 | NILSON, Mia | SO | Windsor (Imperial) High School | 28.80 | Verified |
| 3 | PENDLETON, Jayden | 9 | North County High School | 28.54 | Verified |
| 4 | MUELLERSMAN, Elsa | 9 | Festus High School | 28.32 | Verified |
| 5 | GIVENS, ESSENCE | SO | Sikeston High School | 28.44 | Verified |
| 6 | WATKINS, Amazia | JR | Central (Cape Girardeau) High Sc | 28.76 | Verified |
| 7 | SMITH, Aymerie | JR | Affton High School | 29.30 | Verified |
| 8 | GUSEMAN, Mariah | JR | Windsor (Imperial) High School | 31.16 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | QUARIA METTY, Don | 9 | Sikeston High School | 27.89 | Verified |
| 2 | MILLER, Krysta | JR | Hillsboro High School | 27.41 | Verified |
| 3 | BIEHLE, Alexis | SR | Festus High School | 27.08 | Verified |
| 4 | BOURN, Neoles | SO | DeSoto High School | 26.18 | Verified |
| 5 | MELLOR, Meghan | SR | DeSoto High School | 26.33 | Verified |
| 6 | WENGERT, Hallee | SR | Perryville High School | 27.17 | Verified |
| 7 | KESTER, Felicity | SR | Notre Dame (Cape Girardeau) High | 27.83 | Verified |
| 8 | STROHKIRCH, Cheyenne | SO | Farmington High School | 28.21 | Verified |