| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SCHRAM, Ian | SR | Festus High School | 1:55.61 | Verified |
| 2 | ALLISON, Josh | SR | Hillsboro High School | 1:55.97 | Verified |
| 3 | KRIEG, Cullen | SR | Festus High School | 1:56.38 | Verified |
| 4 | ALLISON, Jonah | SR | Hillsboro High School | 1:56.53 | Verified |
| 5 | SEABAUGH, Ryan | SR | Central (Cape Girardeau) High Sc | 1:56.89 | Verified |
| 6 | FULLER, Evan | JR | Farmington High School | 1:57.57 | Verified |
| 7 | MANN, Orville | SR | Hillsboro High School | 1:57.97 | Verified |
| 8 | RUSTEN, Henry | SR | Central (Cape Girardeau) High Sc | 2:01.08 | Verified |
| 9 | MORALES, Kevin | 9 | Affton High School | 2:08.49 | Verified |
| 10 | ROLAND, Robert | SR | DeSoto High School | 2:10.25 | Verified |
| 11 | BROWN, Micah | 9 | Perryville High School | 2:16.46 | Verified |
| 12 | TWIDWELL, Clay | 9 | Farmington High School | 2:17.39 | Verified |
| 13 | HOECKELE, Porter | SO | Perryville High School | 2:17.46 | Verified |
| 14 | LOVE, Kendarrious | SO | Sikeston High School | 2:18.78 | Verified |
| 15 | CALDWELL, Nikolas | 9 | Windsor (Imperial) High School | 2:20.11 | Verified |
| 16 | SCOTT, COLIN | SO | Sikeston High School | 2:20.99 | Verified |
| 17 | OMEROVIC, Emrah | SR | Affton High School | NT | Unverified: |
| 18 | MORAVEC, Scott | SO | Windsor (Imperial) High School | NT | Unverified: |