| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | EFTINK, Lauren | JR | Notre Dame (Cape Girardeau) High | 2:22.75 | Verified |
| 2 | DAUGHTRY, Anna | SO | Affton High School | 2:24.12 | Verified |
| 3 | THURMAN, Katelyn | 9 | Festus High School | 2:27.20 | Verified |
| 4 | HOLMES, Kaylea | SR | Festus High School | 2:27.91 | Verified |
| 5 | WOOD, Chloe | SR | Farmington High School | 2:29.51 | Verified |
| 7 | CROSBY, Anna | SR | Hillsboro High School | 2:33.78 | Verified |
| 8 | WIETHOP, Katelyn | 9 | Hillsboro High School | 2:34.97 | Verified |
| 9 | ESSNER, Sydney | 9 | Notre Dame (Cape Girardeau) High | 2:42.67 | Verified |
| 10 | KOEHLER, Avery | JR | Central (Cape Girardeau) High Sc | 2:44.56 | Verified |
| 11 | HELLWEGE, Heidi | 9 | Central (Cape Girardeau) High Sc | 2:46.64 | Verified |
| 12 | HENDERSON, Wyllow | 9 | Farmington High School | 2:47.41 | Verified |
| 13 | MULLINS, Sophia | SO | DeSoto High School | 2:47.44 | Verified |
| 14 | FITZGERALD, MORGAN | SR | Sikeston High School | 2:49.59 | Verified |
| 15 | DETERS, Cam | 9 | Windsor (Imperial) High School | 2:52.46 | Verified |
| 16 | LIN, CHLOE | JR | Sikeston High School | 2:55.21 | Verified |
| 17 | BOYSTER, Katie | JR | Windsor (Imperial) High School | 3:02.78 | Verified |