| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ALFONSO, Devin | 9 | Doniphan High School | 51.50 | Verified |
| 2 | POLLEY, Keagan | SO | Dexter High School | 47.71 | Verified |
| 3 | HENDERSON, Connor | SO | Central (New Madrid County) High | 47.19 | Verified |
| 4 | SENSEL, Dane | SO | Fredericktown High School | 46.83 | Verified |
| 5 | SNIDER, Jaxon | JR | Kennett High School | 47.11 | Verified |
| 6 | JOKERST, Hawthorne | JR | Ste. Genevieve High School | 47.63 | Verified |
| 7 | AGUAYO, Robert | 9 | Kelly High School | 50.75 | Verified |
| 8 | PABLO AVILA HENAO, Juan | 9 | Notre Dame (Cape Girardeau) High | 53.62 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | FLORES, Jose | SR | Kennett High School | 44.96 | Verified |
| 2 | SPROUS, Landon | JR | Potosi High School | 42.50 | Verified |
| 3 | FARMER, RJ | JR | Dexter High School | 41.70 | Verified |
| 4 | DOW, Davis | SR | Notre Dame (Cape Girardeau) High | 41.00 | Verified |
| 5 | BOCKENKAMP, Charles | SR | Central (Park Hills) High School | 41.44 | Verified |
| 6 | CROSS, Jarren | JR | Potosi High School | 42.16 | Verified |
| 7 | MCCLELLAN, Evan | JR | Central (New Madrid County) High | 43.88 | Verified |
| 8 | SHOAF, Evan | 9 | Kelly High School | 45.95 | Verified |