| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | JACKSON, Quante | SR | Sikeston High School | 55.03 | Verified |
| 2 | JOHNSON, Wyatt | SO | Festus High School | 45.72 | Verified |
| 3 | SHIRK, Brenton | JR | Windsor (Imperial) High School | 44.44 | Verified |
| 4 | HILL, Landon | JR | DeSoto High School | 43.86 | Verified |
| 5 | BLASSINGAME, Claytin | JR | Windsor (Imperial) High School | 44.17 | Verified |
| 6 | GARRIS, Aden | JR | Perryville High School | 44.52 | Verified |
| 7 | PFAFF, Xavier | SO | Perryville High School | 50.15 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WOODSON, Samuel | JR | Farmington High School | 43.24 | Verified |
| 2 | ALLEN, Elliot | SO | Central (Cape Girardeau) High Sc | 42.20 | Verified |
| 3 | LOGAN, Bryson | SO | Farmington High School | 41.21 | Verified |
| 4 | BROWN, Clayton | 9 | Hillsboro High School | 40.98 | Verified |
| 5 | MARCHETTI, Nick | JR | Hillsboro High School | 41.05 | Verified |
| 6 | BROOKS-DAVIS, Jamauri | SR | Central (Cape Girardeau) High Sc | 42.12 | Verified |
| 7 | PATTERSON, Ethan | SR | DeSoto High School | 42.65 | Verified |
| 8 | WILSON, Richard | SO | Festus High School | 43.54 | Verified |