| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Hillsboro High School | A | Allison | Allison | Belcher | Mann | 7:59.58 | Verified |
| 2 | Festus High School | A | Schram | Riney | Rhine | Uding | 8:05.17 | Verified |
| 3 | Central (Cape Girardeau) High Sc | A | Rusten | Schlick | Seabaugh | Seabaugh | 8:28.09 | Verified |
| 4 | Farmington High School | A | Cain | Randazzo | Kimbrough | Fuller | 8:42.37 | Verified |
| 5 | Affton High School | A | Lovelace | Morales | Pashkov | Mooney | 8:43.24 | Verified |
| 6 | Windsor (Imperial) High School | A | Hurst | Johnson | Moravec | Caldwell | 8:43.95 | Verified |
| 7 | Perryville High School | A | Luckey | Brown | Hoeckele | Hoeckele | 9:04.63 | Verified |
| 8 | Sikeston High School | A | Couch | Love | Applewhite | SCOTT | 9:44.94 | Verified |