| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SHAFFER, Michelle | SO | Affton High School | 1:02.16 | Verified |
| 2 | CELLA, Emelia | SO | Affton High School | 1:00.30 | Verified |
| 3 | JARNEGAN, Hannah | SO | Windsor (Imperial) High School | 55.12 | Verified |
| 4 | SADLER, Nicole | 9 | Central (Cape Girardeau) High Sc | 54.76 | Verified |
| 5 | BLOODWORTH, Angela | JR | Festus High School | 54.98 | Verified |
| 6 | HENNEMANN, Anna | SR | Notre Dame (Cape Girardeau) High | 57.87 | Verified |
| 7 | WILLIAMS, SHANELL | SO | Sikeston High School | 1:00.63 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CASSIMATIS, Carissa | SR | Farmington High School | 51.83 | Verified |
| 2 | FARRAR, Mya | SO | Central (Cape Girardeau) High Sc | 51.14 | Verified |
| 3 | MOORE, Janaye | JR | Sikeston High School | 50.20 | Verified |
| 4 | WRIGHT, Emily | SO | Hillsboro High School | 47.78 | Verified |
| 5 | HAWKINS, Jessica | 9 | Festus High School | 48.53 | Verified |
| 6 | BRANDENBURG, Erin | SO | Hillsboro High School | 51.07 | Verified |
| 7 | CARR, Lily | SO | Notre Dame (Cape Girardeau) High | 51.28 | Verified |
| 8 | HAYWARD, Niyah | 9 | Farmington High School | 54.03 | Verified |