| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | PATEL, Hannah | 9 | Kennett High School | 1:18.97 | Verified |
| 4 | FIGUEROA, Lena | 9 | Kelly High School | 1:13.25 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ROBINSON, Garyn | 9 | Kennett High School | 1:09.77 | Verified |
| 2 | NEA, Meredith | 9 | Dexter High School | 1:07.99 | Verified |
| 3 | MANESS, Macye | SO | Doniphan High School | 1:05.31 | Verified |
| 4 | CLEMENTS, Kale | SO | Ste. Genevieve High School | 59.35 | Verified |
| 5 | ENGLAND, Allie | SO | Central (Park Hills) High School | 1:02.97 | Verified |
| 6 | MOONEY, Cecilia | 9 | Ste. Genevieve High School | 1:07.11 | Verified |
| 7 | NEWSTEAD-ADAMS, Cate | JR | Arcadia Valley High School | 1:08.77 | Verified |
| 8 | FEES, Marie | JR | Dexter High School | 1:13.16 | Verified |