| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | TORRES, Selah | SO | Sikeston High School | 1:12.46 | Verified |
| 3 | HILTON, Ashley | 9 | Hillsboro High School | 1:07.56 | Verified |
| 4 | BURGAN, Elizabeth | JR | Farmington High School | 1:07.35 | Verified |
| 5 | DANE, Bailee | 9 | Farmington High School | 1:07.50 | Verified |
| 6 | WALDEN, Addison | 9 | Festus High School | 1:07.73 | Verified |
| 7 | CRAWFORD, SARAH | SR | Sikeston High School | 1:12.75 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | COUSINS, Addison | 9 | Affton High School | 1:06.98 | Verified |
| 2 | BUETTNER, Sidney | SR | Hillsboro High School | 1:04.10 | Verified |
| 3 | CRADY, Autymn | SO | DeSoto High School | 1:03.43 | Verified |
| 4 | MACKINS, Amiyah | SO | Central (Cape Girardeau) High Sc | 1:00.42 | Verified |
| 5 | DAUGHTRY, Anna | SO | Affton High School | 1:02.05 | Verified |
| 6 | MILLER, Kayla | 9 | North County High School | 1:03.57 | Verified |
| 7 | BROTHERTON, Lily | 9 | Notre Dame (Cape Girardeau) High | 1:05.65 | Verified |
| 8 | PERRY, Presley | SR | Festus High School | 1:07.18 | Verified |