| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Ste. Genevieve High School | A | Jokerst | Merriman | Vogt | Brooks | 50.46 | Verified |
| 2 | Central (New Madrid County) High | A | Minner | Newsom | Bennett | Jones | 45.43 | Verified |
| 3 | Central (Park Hills) High School | A | Wagner | Welch | Petty | Harlow | 44.54 | Verified |
| 4 | Kennett High School | A | Rodgers | Pittman | Boatman | Gaines-Parker | 43.31 | Verified |
| 5 | Notre Dame (Cape Girardeau) High | A | Bruenderman | Dennis | Dow | Brown | 43.61 | Verified |
| 6 | Dexter High School | A | McDonald | Bryant | CUEVAS | Hinson-Flores | 44.60 | Verified |
| 7 | Kelly High School | A | Hileman | Appleton | Burleson | Chapman | 46.84 | Verified |