| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | North County High School | A | McCoy | GOAD | FORNEY | Peniston | 45.53 | Verified |
| 2 | Central (Cape Girardeau) High Sc | A | Murray | Eanes | Clemons | Edwards | 44.74 | Verified |
| 3 | Sikeston High School | A | HOBBS | JACKSON | Artis | KELLUM | 44.62 | Verified |
| 4 | Festus High School | A | Bates | Johnson | Branch | Edwards | 42.59 | Verified |
| 5 | Hillsboro High School | A | Walther | Lutes | Marchetti | Larson | 43.42 | Verified |
| 6 | Farmington High School | A | Brooks | Dickinson | McClain | Berry | 44.69 | Verified |
| 7 | Perryville High School | A | Rhyne | Garris | Hill | Litterst | 45.03 | Verified |
| 8 | Windsor (Imperial) High School | A | Hagan | Patrick | Hefele | Shirk | 45.74 | Verified |