| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Ste. Genevieve High School | A | Fernow | Gross | Mitchell | Vaeth | 56.41 | Verified |
| 3 | Kennett High School | A | Farmer | Gray | Moore | Harrell | 54.31 | Verified |
| 4 | Central (Park Hills) High School | A | Dortch | Scott | Johnson | England | 52.83 | Verified |
| 5 | Dexter High School | A | Krapf | Essner | Kennedy | Stromas | 53.43 | Verified |
| 6 | Kelly High School | A | Ray | King | Johnson | Barnhouse | 56.04 | Verified |