| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 4 | North County High School | A | Lange | McFerron | Karsch | Arnold | 53.47 | Verified |
| 5 | Affton High School | A | Cella | Karahasanovic | Wagstaff | Olesia | 57.17 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Notre Dame (Cape Girardeau) High | A | Kester | Givens | Carr | Tatum | 52.25 | Verified |
| 2 | Farmington High School | A | Cassimatis | Dunn | Shuburt | Strohkirch | 51.96 | Verified |
| 3 | DeSoto High School | A | Missey | Smetzer | Mellor | Bourn | 51.17 | Verified |
| 4 | Central (Cape Girardeau) High Sc | A | Watkins | Williams | Jackson | Bogan | 50.79 | Verified |
| 5 | Sikeston High School | A | Shabazz | FAIRHURST | Quaria Metty | STEWARD | 50.95 | Verified |
| 6 | Festus High School | A | Biehle | Gillam | DeClue | Hawkins | 51.44 | Verified |
| 7 | Windsor (Imperial) High School | A | Jarnegan | Krause | Nilson | Williams | 52.23 | Verified |
| 8 | Hillsboro High School | A | Mora | Buettner | Holland | Brandenburg | 52.91 | Verified |