| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Ste. Genevieve High School | A | Greminger | Zangaro | Klump | Clements | 10:36.37 | Verified |
| 2 | Potosi High School | A | Sansegraw | Gibson | Land | Skiles | 10:48.51 | Verified |
| 3 | Kelly High School | A | Figueroa | Merrick | Essner | Thurmond | 11:18.07 | Verified |
| 4 | Central (Park Hills) High School | A | Voepel | Sharp | Stephens | Hites | 11:32.25 | Verified |
| 5 | Dexter High School | A | Bolin | Engler | Lloyd | Hale | 12:08.65 | Verified |
| 6 | Kennett High School | A | Kamper | Patel | Robinson | Rose | 13:21.66 | Verified |