| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SEABAUGH, Ryan | SR | Central (Cape Girardeau) High Sc | 4:15.78 | Verified |
| 2 | ALLISON, Josh | SR | Hillsboro High School | 4:17.27 | Verified |
| 3 | SCHRAM, Ian | SR | Festus High School | 4:17.91 | Verified |
| 4 | ALLISON, Jonah | SR | Hillsboro High School | 4:19.86 | Verified |
| 5 | MANN, Orville | SR | Hillsboro High School | 4:23.27 | Verified |
| 6 | KRIEG, Cullen | SR | Festus High School | 4:23.30 | Verified |
| 7 | FULLER, Evan | JR | Farmington High School | 4:29.43 | Verified |
| 8 | CHRISTOPHER, Drew | SR | North County High School | 4:49.54 | Verified |
| 9 | AUBUCHON, James | 9 | DeSoto High School | 4:49.99 | Verified |
| 10 | MORAVEC, Scott | SO | Windsor (Imperial) High School | 4:56.54 | Verified |
| 11 | LUCKEY, Kayd | 9 | Perryville High School | 4:56.80 | Verified |
| 12 | SEABAUGH, Jackson | 9 | Central (Cape Girardeau) High Sc | 4:57.52 | Verified |
| 13 | MITCHELL, KALYB | JR | DeSoto High School | 5:00.02 | Verified |
| 14 | LOVE, Kendarrious | SO | Sikeston High School | 5:04.54 | Verified |
| 15 | GALCZYNSKI, Trent | SR | Farmington High School | 5:12.91 | Verified |
| 16 | HOPKINS, Bair | SO | Perryville High School | 5:14.36 | Verified |
| 18 | LANG, Connor | SO | Affton High School | 5:33.92 | Verified |
| 19 | OMEROVIC, Emrah | SR | Affton High School | 5:35.10 | Verified |
| 20 | COUCH, Jackson | 8 | Sikeston High School | 6:12.22 | Verified |