| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SANSEGRAW, Celeste | SR | Potosi High School | 5:36.20 | Verified |
| 2 | MERRICK, Morgan | SO | Kelly High School | 6:01.56 | Verified |
| 3 | GREMINGER, Sadie | SO | Ste. Genevieve High School | 6:09.03 | Verified |
| 4 | LAND, Allison | SO | Potosi High School | 6:11.83 | Verified |
| 5 | HALE, Kali | SO | Dexter High School | 6:13.10 | Verified |
| 6 | VAZQUEZ, Vanessa | JR | East Prairie High School | 6:13.13 | Verified |
| 7 | MEDOWS, Dakotah | SR | Ste. Genevieve High School | 6:17.23 | Verified |
| 8 | CROUCH, Elizabeth | SR | Fredericktown High School | 6:21.90 | Verified |
| 9 | HITES, Jaysa | SO | Central (Park Hills) High School | 6:41.99 | Verified |
| 10 | GARLAND, Patience | SR | Fredericktown High School | 6:52.14 | Verified |
| 11 | BOLIN, Emily | 9 | Dexter High School | 7:07.25 | Verified |
| 12 | RICE, Joslin | SO | Doniphan High School | 8:16.51 | Verified |
| 13 | KAMPER, Emory | 9 | Kennett High School | 8:51.11 | Verified |