| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | EFTINK, Lauren | JR | Notre Dame (Cape Girardeau) High | 5:16.27 | Verified |
| 2 | LEFTWICH, Ava | SR | Festus High School | 5:26.17 | Verified |
| 3 | MORIARTY, Aiden | SR | Farmington High School | 5:29.16 | Verified |
| 4 | WOOD, Chloe | SR | Farmington High School | 5:29.33 | Verified |
| 5 | BISHOP, Tegan | SR | Perryville High School | 5:29.86 | Verified |
| 6 | COLE, Olivia | 9 | Festus High School | 5:33.71 | Verified |
| 7 | CROSBY, Anna | SR | Hillsboro High School | 5:36.04 | Verified |
| 8 | TURNER, Molly | SR | Notre Dame (Cape Girardeau) High | 5:42.98 | Verified |
| 9 | SNIDER, Reagan | SR | Central (Cape Girardeau) High Sc | 6:03.76 | Verified |
| 10 | CALDWELL, Aimee | SO | Central (Cape Girardeau) High Sc | 6:05.27 | Verified |
| 11 | LIN, LUCY | JR | Sikeston High School | 7:15.39 | Verified |