| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 4 | North County High School | A | Peniston | GOAD | GANTZ | FORNEY | DNS | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Sikeston High School | A | HOBBS | Artis | WILKINS | KELLUM | 1:34.03 | Verified |
| 2 | Affton High School | A | Lovelace | Mitchell | Pashkov | Williams | 1:33.82 | Verified |
| 3 | Central (Cape Girardeau) High Sc | A | Murray | Clemons | Brooks-Davis | Edwards | 1:30.05 | Verified |
| 4 | Hillsboro High School | A | Marchetti | Lutes | Larson | Voyles | 1:28.78 | Verified |
| 5 | Festus High School | A | Johnson | Branch | Edwards | Bates | 1:29.68 | Verified |
| 6 | DeSoto High School | A | Bourn | Missey | SISSON | Pirtle | 1:33.31 | Verified |
| 7 | Windsor (Imperial) High School | A | Becker | Hollis | Patrick | Hagan | 1:34.00 | Verified |
| 8 | Farmington High School | A | Randazzo | Drye | McClain | Berry | 1:34.19 | Verified |