| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Kelly High School | A | Ray | King | Klipfel | Barnhouse | 2:04.63 | Verified |
| 3 | Dexter High School | A | Krapf | Essner | Nea | Turnbo | 1:54.03 | Verified |
| 4 | Central (Park Hills) High School | A | Dortch | Scott | Johnson | England | 1:52.71 | Verified |
| 5 | Ste. Genevieve High School | A | Mooney | Kohler | Merklin | Jerman | 1:53.40 | Verified |
| 6 | Kennett High School | A | Heeb | Moore | Gray | Farmer | 1:55.30 | Verified |