| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 4 | Notre Dame (Cape Girardeau) High | A | Huck | Seyer | White | Hennemann | 1:54.69 | Verified |
| 5 | North County High School | A | Layne | Pendleton | Miller | McFerron | 1:56.00 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Farmington High School | A | Cassimatis | Dunn | Shuburt | Strohkirch | 1:52.75 | Verified |
| 2 | Windsor (Imperial) High School | A | Dixon | Krause | Mancuso | Mancuso | 1:51.55 | Verified |
| 3 | DeSoto High School | A | Missey | Mitkos | Mellor | Bourn | 1:47.47 | Verified |
| 4 | Central (Cape Girardeau) High Sc | A | Williams | Bogan | Jackson | Mackins | 1:45.96 | Verified |
| 5 | Festus High School | A | Biehle | Gillam | DeClue | Hawkins | 1:47.10 | Verified |
| 6 | Sikeston High School | A | GIVENS | Moore | Shabazz | Quaria Metty | 1:51.15 | Verified |
| 7 | Hillsboro High School | A | Mora | Miller | Holland | Nichols | 1:51.94 | Verified |
| 8 | Affton High School | A | Smith | Daughtry | Cousins | Wilson | 1:54.66 | Verified |