| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CURD, Kensington | FR | Brentwood High School | 5:44.21 | Verified |
| 2 | HENRY, Destiny | SO | University City High School | 5:46.11 | Verified |
| 3 | WENGER, Madeline | SO | Rosati-Kain High School | 6:16.41 | Verified |
| 4 | BRADFORD, Brianna | SO | Bayless High School | 6:49.76 | Verified |
| 5 | ZEWKI, Amelia | FR | Bayless High School | 7:07.97 | Verified |
| 6 | IMBULA, Gabriella | FR | Collegiate School of Medicine an | 7:48.32 | Verified |