| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | University City High School | A | Armour-Dozier | Black | Cooley | Hill | 1:37.35 | Verified |
| 3 | Bayless High School | A | Miller | Patton | Jones | Uebari | 1:35.03 | Verified |
| 4 | Brentwood High School | A | Hawkins | Lane | Phillips | Thompson | 1:36.68 | Verified |
| 5 | Hancock High School | A | Martin | Miner | Stewart | Wise | NT | Verified |