| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Gateway Science Charter | A | Alnusour | Axe | Jeltes | Spieckerman | 4:12.52 | Verified |
| 3 | University City High School | A | Armour-Dozier | Black | Sims | Watt Hoven | 3:50.35 | Verified |
| 4 | Bayless High School | A | Miller | Ibrahim | Saville | Hulsey | 3:58.97 | Verified |