| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SCARBROUGH, Makyah | JR | Bayless High School | NT | Unverified: |
| 2 | PRATER, A'maya | SO | Valley Park High School | 1:17.41 | Verified |
| 3 | BOHLEN, Kornae | SO | Collegiate School of Medicine an | NT | Unverified: |
| 4 | PRICE, Kaniya | SO | Collegiate School of Medicine an | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | JUGOVIC, Selma | SO | Bayless High School | 55.92 | Verified |
| 2 | DIXON, Laila | JR | University City High School | 51.84 | Verified |
| 3 | ELSTON, Brooke | SR | Rosati-Kain High School | 54.48 | Verified |
| 4 | GARCIA-PINTOR, Joy | SO | Gateway Science Charter | 59.09 | Verified |