| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | University City High School | A | Robinson | Cooley | Jennings | Hill | 46.97 | Verified |
| 2 | Valley Park High School | A | 46.40 | Verified | ||||
| 3 | Bayless High School | A | Sestan | Uebari | Jones | Patton | 45.42 | Verified |
| 4 | Brentwood High School | A | Harris | Hawkins | Clay | Lane | 45.61 | Verified |
| 5 | Hancock High School | A | Miner | Hurd | Whitelow | Wise | 46.60 | Verified |