| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | St. Francis Borgia High School | A | Mohesky | Hoer | Kell | Mitchell | NT | Unverified: |
| 3 | Wright City High School | B | NT | Unverified: | ||||
| 4 | Helias Catholic High School | B | Tharp | Schatzer | Jerozal | Neiman | 1:33.34 | Verified |
| 5 | Helias Catholic High School | A | Hoelscher | Neuenswander | Crisostomo | Lanigan | 1:33.34 | Verified |
| 6 | St. Francis Borgia High School | B | Schumacher | Glosemeyer | Shoemaker | Jones | NT | Unverified: |
| 7 | Wright City High School | A | NT | Unverified: |