| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Sullivan High School | A | Denney | Brendel | Beagle | Ray | NT | Unverified: |
| 3 | St. Clair High School | A | Sohn | Folks | Bekemeier | Sikes | NT | Unverified: |
| 4 | Helias Catholic High School | A | Benne | Neuenswander | Crisostomo | Swan | 3:37.91 | Verified |
| 5 | Helias Catholic High School | B | Hamilton | Loesch | Lamb | Warner | 3:37.91 | Verified |
| 6 | Wright City High School | A | NT | Unverified: | ||||
| 7 | Wright City High School | B | NT | Unverified: |