| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Helias Catholic High School | B | Wieberg | Borgmeyer | Grothoff | Voegeli | 8:35.47 | Verified |
| 2 | Helias Catholic High School | A | Doolittle | Ammons | Voss | Loesch | 8:35.47 | Verified |
| 3 | Sullivan High School | A | Roberts | Denney | Whaley | Studdard | NT | Unverified: |
| 4 | St. Clair High School | B | Adams | Krouper-Martinez | Slater | Souza | NT | Unverified: |
| 5 | St. Clair High School | A | James | Kern | Perkins | Yarberry | NT | Unverified: |
| 6 | St. Francis Borgia High School | A | Wolfe | Dunard | Mort | Posinski | NT | Unverified: |
| 7 | St. Francis Borgia High School | B | NT |